Standard Methods Research Articles

System dynamics applied in enterprise engineering – a systematic literature review

Purpose This study aims to investigate the combined use of System Dynamics (SD) applications in Enterprise Engineering (EE) research and practice. SD application in EE is becoming widely accepted as a tool to support decision-making processes and for capturing relationships within enterprises. Design/methodology/approach A systematic literature review (SLR) is conducted using a standard SLR method to provide a comprehensive review of existing literature. The search was conducted on ten platforms identifying 30 publications which were analysed through the use and development of a codebook. Findings The SLR showed that 90% of the result set consisted of peer-reviewed academic conferences and journal papers. The SLR identified a highly dispersed author set of 83 authors. Amongst these authors, Vinay Kulkarni was an active author who has co-authored up to four publications in this research area. The analysis further revealed that the combined use of SD applications and EE is an emerging research area that still needs to develop in maturity. While all phases of EE have received attention, the current research work is more focused on the design phase. The important gap between model development and implementation is identified. Originality/value The study elucidates the existing status of interdisciplinary research combining techniques from the SD and EE disciplines, suggesting future research topics that combine the strengths of these existing disciplines.

Safety of Endoscopic Procedures in Thrombocytopenia and Neutropenia: Embracing Assurance.

Few studies have evaluated the post-endoscopic adverse events in patients with neutropenia and thrombocytopenia. Current guidelines do not provide clear direction on this topic. We explore the pooled rates of safety and adverse effects of endoscopic interventions in thrombocytopenia and neutropenia patients via a systematic review & meta-analysis. Databases, including Medline, Scopus, and Embase, were searched (in May 2023) using specific terms for studies evaluating the clinical outcomes of endoscopy in patients with thrombocytopenia and neutropenia. Standard meta-analysis methods were employed using the random-effects model. I2% heterogeneity was used to assess the heterogeneity. Six studies and four studies evaluated endoscopic outcomes in patients with thrombocytopenia and neutropenia respectively with mean age was 56years. The pooled rate of total post-biopsy bleeding and total post-polypectomy bleeding among patients with thrombocytopenia was 4% (95% CI 1-11), I2 = 84%, and 12% (95% CI 3-36) I2 = 43%. The total rate of post procedure-related bleeding in thrombocytopenia was 5% (95% CI 1-14) I2 = 95%. The pooled rate of post-endoscopic infection (fever from any cause, bacteremia) in neutropenia was 10% (95% CI 3-28%) I2 = 96%. On sub analysis, the pooled rate of bacteremia and 30days all-cause mortality in neutropenia was 4% (95% CI 3-5%) I2 = 0% and 13% (95% CI 4-34%) I2 = 95% respectively. Our data supports the notion that endoscopic procedures are safe for neutropenic, thrombocytopenic patients with suitable indications and reasonable functional status and have an acceptable risk/benefit ratio.

Exosomes: the next-generation therapeutic platform for ischemic stroke

Abstract Current therapeutic strategies for ischemic stroke fall short of the desired objective of neurological functional recovery. Therefore, there is an urgent need to develop new methods for the treatment of this condition. Exosomes are natural cell-derived vesicles that mediate signal transduction between cells under physiological and pathological conditions. They have low immunogenicity, good stability, high delivery efficiency, and the ability to cross the blood-brain barrier. These physiological properties of exosomes have the potential to lead to new breakthroughs in the treatment of ischemic stroke. The rapid development of nanotechnology has advanced the application of engineered exosomes, which can effectively improve targeting ability, enhance therapeutic efficacy, and minimize the dosages needed. Advances in technology have also driven clinical translational research on exosomes. In this review, we describe the therapeutic effects of exosomes anc their positive roles in current treatment strategies for ischemic stroke, including their anti-inflammation, anti-apoptosis, autophagy-regulation, angiogenesis, neurogenesis, and glial scar formation reduction effects. However, it is worth noting that, despite their significant therapeutic potential, there remains a dearth of standardized characterization methods and efficient isolation techniques capable of producing highly purified exosomes. Future optimization strategies should prioritize the exploration of suitable isolation techniques and the establishment of unified workflows to effectively harness exosomes for diagnostic or therapeutic applications in ischemic stroke. Ultimately, our review aims to summarize our understanding of exosome-based treatment prospects in ischemic stroke and foster innovative ideas for the development of exosome-based therapies.

Development of an International Standard Set of Outcomes and Measurement Methods for Routine Practice for Adults with Epilepsy: The International Consortium for Health Outcomes Measurement Consensus Recommendations.

At present, there is no internationally accepted set of core outcomes or measurement methods for epilepsy clinical practice. Therefore, the International Consortium for Health Outcomes Measurement (ICHOM) convened an international working group of experts in epilepsy, people with epilepsy and their representatives to develop minimum sets of standardized outcomes and outcomes measurement methods for clinical practice that support patient-clinician decision-making and quality improvement. Consensus methods identified 20 core outcomes. Measurement tools were recommended based on their evidence of strong clinical measurement properties, feasibility, and cross-cultural applicability. The essential outcomes included many non-seizure outcomes: anxiety, depression, suicidality, memory and attention, sleep quality, functional status, and the social impact of epilepsy. The proposed set will facilitate the implementation of the use of patient-centered outcomes in daily practice, ensuring holistic care. They also encourage harmonization of outcome measurement, and if widely implemented should reduce the heterogeneity of outcome measurement, accelerate comparative research, and facilitate quality improvement efforts.

Experimental design approach for the quantitative analysis of multicomponents by single marker and HPLC fingerprinting of Thunbergia laurifolia aqueous extract.

Thunbergia laurifolia is used in traditional Thai medicine to reduce fever and treat mouth ulcers. However, the quantitative analysis of chemical markers has not yet been officially defined. The objective of this study is to develop a high-performance liquid chromatography (HPLC) method using a design of experiment (DoE) for the quantitative analysis of multicomponents by single marker (QAMS) and fingerprinting of the T. laurifolia aqueous extract. Critical variables were screened using a two-level fractional factorial design, followed by the optimization of the selected variables using a central composite design. The validated method was applied for quality assessment based on QAMS and fingerprinting of the extract. Optimum conditions of DoE for the analysis of caffeic acid, vicenin-2, and rosmarinic acid were determined. The relative correction factors for caffeic acid and vicenin-2 were calculated using rosmarinic acid as an internal reference standard, and their contents in 30 samples were determined. The differences between the external standard method (ESM) and QAMS were compared. No significant difference was observed in the quantitative determination, proving the consistency QAMS and ESM. HPLC fingerprints of T. laurifolia were established with 8 of 12 characteristic peaks that were structurally characterized using HPLC-diode array detection-electrospray ionization/tandem mass spectrometry. The similarity of the fingerprints in all samples was ≥0.74, and the pattern recognition of the characteristic peaks was satisfied. The proposed method efficiently detected multiple components of the T. laurifolia extract. Thus, the method is beneficial in providing references for enhancing the quality control of other herbal medicines.

Investigation of the influence of walnut shell extract on chromium (VI) content in leather

The effect of walnut shell extract ( Juglans regia) on chromium (VI) formation in leather during the finishing process is investigated in this study. The walnut shell extract composition was determined using gas chromatography. To enable chromium (VI) formation, finished leather samples were aged under various conditions, including exposure to ultraviolet light. The amount of chromium (VI) in each leather sample was measured using a Shimadzu UV-1601 PC UV-visible spectrophotometer at 540 nm based on the ISO/FDIS 17075 standard method. The results showed that the extracts from walnut shell ( Juglans regia) significantly reduced chromium (VI) formation in the leather under all aging conditions.

HANS GEORG GADAMER'S HERMENEUTICS VS BIBLICAL HERMENEUTICS: A COMPARATIVE ANALYSIS

At first, Hermeneutics as an interacting science was only used among the church as a method of Scripture Interpretation, with a standard system or method of hermeneutics. This hermeneutics is often referred to as Traditional Hermeneutics or Biblical Hermeneutics. However, in its development, Hermeneutics came out of biblical studies into the scope of philosophy, art, literature, and history. This hermeneutics is referred to as New Hermeneutics and appears in various variants. The purpose of this article is to introduce the New Hermeneutics system of the Postmodernist era, specifically Hans Georg Gadamer's Hermeneutics, and compare it objectively with the Biblical Hermeneutics System with the standard Grammatical-Historical method, which is often used to interpret the Bible. This study uses the comparative analysis study method to find fundamental similarities and differences between Hans Georg Gadamer's hermeneutic concepts and Biblical hermeneutics. Finally, the author makes conclusions and recommendations for Bible interpreters who are doing hermeneutics.

The Effectiveness of Direct Instruction Model in Teaching Solfeggio for Beginners

The background of this study was rooting down from the students music learning circumstances, of which they have low skill and knowledge of solfeggio, low ability of transferring solfeggio automatically into a new context although they have learned it, unavailability of learning material, syllabus, and lesson plan, standardized method and media of learning. Based on the problems stated above, it is very important to design a research dealing with learning solfeggio which lasting and producing a complete, practical, reliable and valid curricula of solfeggio. The basis model of development is using ADDIE theory. This research belongs to R & D research. The subject of the research is the students of art and music department, FBS Padang State University. The instrumentation of data gathering was using interview, questionnaire, tryout, performance-test. To analyze the raw data to see the level of reliability and practicality the researcher used the formula of Rho Spearman Brown, and the result is that r-obtain 1.2 exceeds r-table within significance level of 95% (0.05) it is 0.349 or by (0,01) it is 0.449. To prove the effectiveness of the product of this research ( the model of learning solfeggio ), the writer distinguish the data of pre-test to post-test by accumulating the scores the students obtained by using match t-test, and the result is that t-obtain 9.311 exceeds t-table 1.697. It means that, the model of learning solfeggio as the product of this research is much more effective significantly since the result of the match t-test is so high exceeding the number of the t-tab. It can be concluded that this model is very useful for the lecturers and the students as well in learning solfeggio at the music department of FBS Padang State University.

Antifungal Potency of Some Antifungal Chemotherapeutics and Antidandruff Solutions against Dermatophytic Flora from Local Hairdressers’ Tools in Awka, Nigeria

Dermatophytes produce spores that can survive for a long time on fomites such as hairdressing tools (combs, scissors, clippers and hairpins). These fomites intend serve in the transmission of dermatophytosis. This study was aimed at evaluating and comparing the antifungal efficacy of some antifungal chemotherapeutics and antidandruff solutions against dermatophytic flora of hairdressers’ tools in Eke-Awka Market in Awka, Anambra State, Nigeria. A total of forty-five (45) samples were collected by swabbing the hairdressing tools of the local hair dressers using sterile swab sticks. The antifungal drugs (Ketoconazole, Miconazole and Griseofulvin) and the antidandruff solutions (Shampoo and Conditioner) were also bought from Eke-Awka Market. The samples where inoculated into sterile Sabouraud Dextrose Broth base in test tubes and incubated at 25-27ºC for 5-14 days. After incubation, the test tubes with visually observed growth (turbidity) were subsequently plated out by streaking on freshly prepared Sabouraud Dextrose Agar supplemented with Chloramphenicol (50µg/ml) and incubated at 25°C for up to 7-14 days. Identification was done using standard methods. Agar well diffusion method was employed for the in vitro antifungal susceptibility testing using different concentrations of both the antifungal drugs and antidandruff solutions obtained using double fold serial dilutions. Twenty Four (24) dermatophytes were isolated and identified. The isolates included Microsporum ferrugineum 6 (25%), Microsporum gypseum 3 (12.5%), Micosporum audouinii 6 (25%), Trichophyton schoenleinii 5 (20.8%) and Trichophyton mentagrophytes 4 (16.7%). The percentage susceptibility pattern of the isolates showed that 60℅ of the isolates were susceptible to Ketoconazole, 40℅ respectively susceptible and susceptible doze dependent to Miconazole and 100℅ resistant to Griseofulvin. For the antidandruff solutions, 20℅ of the isolates were susceptible to hair shampoo while 100℅ were resistant to hair conditioner. The results of this study showed that dermatophytes are prevalent on the tools used by local hair dressers and also that the antifungal drugs had better activity on the isolates than the antidandruff solutions used. Also, based on this study, ketoconazole is recommended for dermatophytosis. There is therefore need to educate the populace on the health hazards associated with sharing local hair making tools and the need for clients to have their personal hair making tools.

Effect of different constraining boundary conditions on simulated femoral stresses and strains during gait

Finite element analysis (FEA) is commonly used in orthopaedic research to estimate localised tissue stresses and strains. A variety of boundary conditions have been proposed for isolated femur analysis, but it remains unclear how these assumed constraints influence FEA predictions of bone biomechanics. This study compared the femoral head deflection (FHD), stresses, and strains elicited under four commonly used boundary conditions (fixed knee, mid-shaft constraint, springs, and isostatic methods) and benchmarked these mechanics against the gold standard inertia relief method for normal and pathological femurs (extreme anteversion and retroversion, coxa vara, and coxa valga). Simulations were performed for the stance phase of walking with the applied femoral loading determined from patient-specific neuromusculoskeletal models. Due to unrealistic biomechanics observed for the commonly used boundary conditions, we propose a novel biomechanical constraint method to generate physiological femur biomechanics. The biomechanical method yielded FHD (< 1 mm), strains (approaching 1000 µε), and stresses (< 60 MPa), which were consistent with physiological observations and similar to predictions from the inertia relief method (average coefficient of determination = 0.97, average normalized root mean square error = 0.17). Our results highlight the superior performance of the biomechanical method compared to current methods of constraint for both healthy and pathological femurs.

Nutritional Characteristics and Sensory Properties of Maize-based Ogi Supplemented with Mushroom (Pleurotus ostreatus) Flour

The study evaluates the effect of supplementation of maize-based ogi with mushroom (Pleurotus ostreatus) flour on nutritional characteristics and sensory properties. Standard processing and analytical methods were used in raw material preparation and analyses. Ogi and mushroom flour were blended in ratio of 100:0, 90:10, 80:20, 70:30 and labeled A, B, C and D respectively. A commercial maize based complementary food was used as control. There was significant difference (p &lt; 0.05) among all samples for all the Vitamin assayed with values ranging from 4.86 to 9.06 (µg/100g), 0.32 to 1.09 mg/100 g, 0.22 to 0.69 mg/100 g, 3.82 to 13.03 mg/100 g and 0.13 to 1.12 mg/100 g for vitamin A, B1, B2, B3 and C. The vitamin values compare favourably with that of control sample. Significant (P&lt;0.05) difference exists across the samples for Zn, Mg, Fe, K and Ca with values ranging from 1.12 to 1.71 mg/100 g, 37.7 to 102 mg/100 g, 4.72 to 13.23 mg/100 g, 23.05 to 125.3 mg/100 g and 125.5 to 160.25 mg/100 g respectively with corresponding higher values for control sample. Result showed negligible amounts of anti-nutritional factors ranging from 0.29 to 0.36 mg/100 g, 0.02 to 0.03 mg/100 g, 0.03 to 0.06 mg/100 g and 0.56 to 0.83 mg/100 g for phytate, oxalate, trypsin inhibitor and tannin respectively. The functional properties of the flour blends values ranged from 0.58 to 0.72 g/mL, 0.99 to 2.2 g/g, 5.21 to 8.94 g/g, 1.75 to 3.72 mL/g and 5.5 to 9.0% for bulk density, water absorption capacity, water retention capacity, swelling capacity and least gelation concentration respectively. Sensory scores revealed substantial overall acceptability of the samples. The quality of ogi samples were greatly improved by mushroom supplementation and could be nutritional beneficial to children and adults.

Bridging Languages through Images: A Multilingual Text-to-Image Synthesis Approach

This research investigates the challenges posed by the predominant focus on English language text-to-image generation (TTI) because of the lack of annotated image caption data in other languages. The resulting inequitable access to TTI technology in non-English-speaking regions motivates the research of multilingual TTI (mTTI) and the potential of neural machine translation (NMT) to facilitate its development. The study presents two main contributions. Firstly, a systematic empirical study employing a multilingual multi-modal encoder evaluates standard cross-lingual NLP methods applied to mTTI, including TRANSLATE TRAIN, TRANSLATE TEST, and ZERO-SHOT TRANSFER. Secondly, a novel parameter-efficient approach called Ensemble Adapter (ENSAD) is introduced, leveraging multilingual text knowledge within the mTTI framework to avoid the language gap and enhance mTTI performance. Additionally, the research addresses challenges associated with transformer-based TTI models, such as slow generation and complexity for high-resolution images. It proposes hierarchical transformers and local parallel autoregressive generation techniques to overcome these limitations. A 6B-parameter transformer pretrained with a cross-modal general language model (CogLM) and fine-tuned for fast super-resolution results in a new text-to-image system, denoted as It, which demonstrates competitive performance compared to the state-of-the-art DALL-E-2. Furthermore, It supports interactive text-guided editing on images, offering a versatile and efficient solution for text-to-image generation.. Keywords: Text-to-image generation, Multilingual TTI (mTTI), Neural machine translation (NMT), Cross-lingual NLP, Ensemble Adapter (ENSAD), Hierarchical transformers, Super- resolution, Transformer-based models, Cross-modal general language model (CogLM).

Impact of financial technologies, digitalization, and natural resources on environmental degradation in G-20 countries: Does human resources matter?

This study assesses the impact of financial technologies, digitalization, natural resources and human resources on environmental degradation in 20 (G-20) countries from 2000 to 2021. It also examines the moderating role of human resources in the relationship between natural resources, digitalization and financial technologies. The study uses the Panel Corrected Standard Error (PCSE) and Method of Moments Quantile (MMQR) regression approaches for the empirical analysis. The results show that digitalization, natural resources and human resources significantly and positively affect environmental degradation, while financial technologies negatively affect environmental degradation in the PCSE estimation. Regarding interaction effects, the study finds that human resources positively affect the relationship between financial technologies and environmental degradation and negatively affect the relationship between natural resources, digitalization and environmental degradation. The MMQR estimation ensures the robustness of these results.

UHVDC transmission line diagnosis method for integrated community energy system based on wavelet analysis

With the large-scale development of renewable energy power, China has faced with the challenges of the reverse regional distribution of wind and solar resources and power load, as well as the intermittency and randomness of renewable energy power. Therefore, China is vigorously developing ultra-high voltage direct current (UHVDC) transmission technology to solve the problem of insufficient flexibility caused by the uncertainty of renewable energy and the fluctuation of multi-energy loads in integrated community energy systems. UHVDC plays an increasingly pivotal role in the west-east transmission system in China’s power system due to its high transmission capacity and long transmission distance. Once the fault occurs in the ultra-high voltage direct (UHVD) transmission line, quick and accurate fault location identification is of great significance. Hence, this paper proposes a UHVDC transmission line diagnosis method based on wavelet analysis for integrated community energy systems. Wavelet transform (WT) is used to decompose the transient signal on a multi-scale, and then power systems computer-aided design (PSCAD) software is utilized for simulation calculation to obtain the singular spectrum entropy of each layer and facilitate wavelet transformations for signal denoising with advanced tools such as MATLAB. The prediction results can distinguish outside the rectification side fault, within the rectification side fault, and outside the inverter fault with an accuracy of 100%. A large number of simulations demonstrate that combining singular spectrum entropy with support vector machines (SVM) has emerged as a robust technique for integrated community energy systems, suggesting its potential as a standard method in UHVDC transmission line diagnosis. This study is of significant reference for realizing the complementarity of multiple types of power supply and ensuring a reliable power supply.

Studies on the Assessment of Hair Cortisol Concentration in Dogs by Radioimmunoassay (RIA)

Background: Hair cortisol Concentration (HCC) is often used as a measure of chronic stress responses in humans. The use of Radioimmunoassay (RIA) and HCC to assess stress in hospitalized dogs has been poorly reported in the literature in India and Central East Asia. This paper presents a modified protocol for measuring HCC in dog hair samples for stress assessment using RIA. Methods: Estimation of HCC concentrations of healthy (n=20) and hospitalized (n=20) dogs was done using RIA. Hair samples were collected from a dog’s nape region and weighed to measure HCC concentration. The study used RIA-enabled dissevered spiking and detectable concentration-based modifications to measure HCC. Five known cortisol concentration standards (viz 19.0 nmol/l, 47.9 nmol/l, 190 nmol/l, 238.66 nmol/l and 713.33 nmol/l) were used, with the first three readily available with a cortisol RIA kit. The samples were spiked with 50 µL of the standard and analysed in a gamma counter to take counts per minute (CPM). The final concentration of HCC was estimated in nmol/l and derived in pg/mg as per standard methods. Result: Healthy dogs had a mean HCC of 6.24±0.84 pg/mg, while hospitalized dogs had 31.65±5.87 pg/mg. A significant deviation was observed in both groups. A box and whisker graph showed an HCC range of 0.36 to 13.78 pg/mg for healthy dogs and 8.70 to 65.25 pg/mg for hospitalized dogs. The interquartile range (25th to 75th percentile) was 3.55 to 9.24 pg/mg for healthy dogs and 14.43 to 46.22 for hospitalized dogs. The observed median for healthy dogs was 6.16 pg/mg and 22.62 pg/mg for hospitalized dogs. The study shows higher HCC in hospitalized sick dogs compared to clinically healthy ones, suggesting hair sample collection as a simple stress assessment method and RIA as a promising HCC detection technique.

Fast and High-Resolution T2 Mapping Based on Echo Merging Plus k-t Undersampling with Reduced Refocusing Flip Angles (TEMPURA) as Methods for Human Renal MRI.

To develop a highly accelerated multi-echo spin-echo method, TEMPURA, for reducing the acquisition time and/or increasing spatial resolution for kidney T2 mapping. TEMPURA merges several adjacent echoes into one k-space by either combining independent echoes or sharing one echo between k-spaces. The combined k-space is reconstructed based on compressed sensing theory. Reduced flip angles are used for the refocusing pulses, and the extended phase graph algorithm is used to correct the effects of indirect echoes. Two sequences were developed: a fast breath-hold sequence; and a high-resolution sequence. The performance was evaluated prospectively on a phantom, 16 healthy subjects, and two patients with different types of renal tumors. The fast TEMPURA method reduced the acquisition time from 3-5 min to one breath-hold (18 s). Phantom measurements showed that fast TEMPURA had a mean absolute percentage error (MAPE) of 8.2%, which was comparable to a standardized respiratory-triggered sequence (7.4%), but much lower than a sequence accelerated by purely k-t undersampling (21.8%). High-resolution TEMPURA reduced the in-plane voxel size from 3 × 3 to 1 × 1 mm2, resulting in improved visualization of the detailed anatomical structure. In vivo T2 measurements demonstrated good agreement (fast: MAPE = 1.3%-2.5%; high-resolution: MAPE = 2.8%-3.3%) and high correlation coefficients (fast: R = 0.85-0.98; high-resolution: 0.82-0.96) with the standardized method, outperforming k-t undersampling alone (MAPE = 3.3-4.5%, R = 0.57-0.59). TEMPURA provides fast and high-resolution renal T2 measurements. It has the potential to improve clinical throughput and delineate intratumoral heterogeneity and tissue habitats at unprecedented spatial resolution.

Teeth pathology of horse from the khirgisuur satellite burials in Orkhon valley

The purpose of this research is to clarify the dental diseases of horses in the Khirgisuur satellite burials of the Late Bronze Age of Mongolia, and to discuss the age and sex of the selected horses. The study included 96 horse bones from #OOR-396th of Khirgisuur satellite burials in the Saikhan Bulgiin denj. The survey was conducted according to standard zooarchaeological methods. According to the results of the research, 35.4% of all horses had tooth decay, jaw inflammation or tumour, and tooth growth defects. It was also observed that young horses were predominantly used for sacrifice. In conclusion, in some cases, choices have been made to support the healthy growth of horses.

Reliable Lift‐Off Patterning of Graphene Dispersions for Humidity Sensors

AbstractDispersion‐based graphene materials are promising candidates for various sensing applications. They offer the advantage of relatively simple and fast deposition via spin‐coating, Langmuir–Blodgett deposition, or inkjet printing. Film uniformity and reproducibility remain challenging in all of these deposition methods. Here, a scalable structuring method is demonstrated, characterized, and successfully applied for graphene dispersions. The method is based on a standard lift‐off process, is simple to implement, and increases the film uniformity of graphene devices. It is also compatible with standard semiconductor manufacturing methods. Two different graphene dispersions are investigated via Raman spectroscopy and Atomic Force Microscopy and observe no degradation of the material properties by the structuring process. Furthermore, high uniformity of the structured patterns and homogeneous graphene flake distribution is achieved. Electrical characterizations show reproducible sheet resistance values correlating with material quantity and uniformity. Finally, repeatable humidity sensing is demonstrated with van der Pauw devices, with sensing limits of less than 1% relative humidity.

A Tissue Distribution Study of Propafenone in an Intentional Fatal Poisoning Case

Propafenone (PPF) belongs to the class 1C antiarrhythmics and can cause electrocardiogram-associated adverse/toxic effects. Cases of PPF intoxication are rarely investigated. We developed a novel and selective GC-MS/MS method for the determination of PPF and its tissue distribution in an intentional fatal poisoning case, which is applicable to PPF quantification in the range of therapeutic to lethal concentrations in complex post-mortem samples. A simple and effective sample pretreatment was applied to all analyzed samples. PPF was determined without the need for dilution, even in highly complex samples containing a wide range of analyte concentrations. Quantification was performed using the standard addition method, developed and validated according to the ICH M10 guidelines. The obtained results indicated that the PPF concentration in the serum from blood taken while alive, before therapy, was the highest ever reported in the literature. Despite the intensive therapy after the patients’ admission, the PPF concentrations in the lungs, spleen, femoral blood and cardiac blood were fatal or abnormally high. On the other hand, the concentrations in the liver and skeletal muscle were lower or approximately the same as observed in cases with therapeutic doses. To the best of our knowledge, the distribution of PPF has not been investigated in fatal intoxication cases and can be helpful in clinical or forensic toxicology.

Blockchain-enabled platform-as-a-service for production management in off-site construction design using openBIM standards

Integrating building information modeling (BIM) with emerging blockchain technology has gained considerable attention in addressing design collaboration problems like poor data security, weak traceability, and low transparency in off-site construction. However, blockchain implementations in off-site construction design, especially with openBIM standards, currently lack standardized workflow management and methods for ensuring data completeness within the blockchain environment. To bridge these gaps, this paper presents a novel blockchain-enabled platform-as-a-service (PaaS), specifically tailored for off-site construction to enhance interoperability and collaborative design efficiency using openBIM standards. The proposed PaaS offers two significant intellectual contributions: (1) a data contract system that manages openBIM workflow standardization and (2) a data escrow system that ensures the completeness and integrity of openBIM data. The data contract system utilizes an innovative asynchronous smart contract algorithm to streamline workflow checks, while the data escrow system introduces a new blockchain-driven data-checking strategy to ensure openBIM completeness at the data level. Besides, a PaaS architecture is developed to clarify the interaction logic between these two systems. The PaaS is validated and evaluated in openBIM-based design collaborations from a real-world project, with results indicating improvements: a 38% time-saving in design issue coordination and a 32% reduction in design data delivery time. Furthermore, the platform received favorable feedback from project participants, validating the approach and confirming the PaaS as a valuable tool for enhancing design collaboration in off-site construction.